Glycoconjugates from high endothelial cells. I. Partial characterization of a sulphated glycoconjugate from the high endothelial cells of rat lymph nodes.

Rat lymph nodes secrete a sulphated macromolecule. This molecule was isolated by ion-exchange chromatography and partially characterized by gel-permeation chromatography, before and after chemical and enzymic modification, and electrophoresis on polyacrylamide gels, paper and glassfibre paper. The results obtained suggest that the label was not incorporated into a proteoglycan but entered a glycolipid that is non-covalently linked to a glycoprotein. A possible role for this complex is discussed.